Device

Part:BBa_K2500008:Design

Designed by: Irma Telarovic   Group: iGEM17_ETH_Zurich   (2017-10-24)


TlpA: Constitutively Expressed Temperature-Dependent Transcriptional Repressor with Engineered RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 870
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1173
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon-optimized for expression in E. coli Nissle 1917.

Source

Organism: Salmonella typhimurium
Original sequence: Piraner, Dan I., et al. "Tunable thermal bioswitches for in vivo control of microbial therapeutics." Nature chemical biology 13.1 (2017): 75-80.
Codon-optimized sequence: gBlock